abstract：:The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms a periodic pattern of nitrogen-fixing heterocysts when grown in the absence of combined nitrogen. PatA is necessary for proper patterning of heterocysts along filaments. In this study, apparent transcriptional start points (tsps) were identified at nucl...

journal_title：Journal of bacteriology

authors： Young-Robbins SS,Risser DD,Moran JR,Haselkorn R,Callahan SM

更新日期：2010-09-01 00:00:00

abstract：:As part of our effort to uncover the molecular basis for the phenotypic variation among clinical Mycobacterium tuberculosis isolates, we have previously reported that isolates belonging to the W/Beijing lineage constitutively overexpress the DosR-regulated transcriptional program. While generating dosR knockouts in tw...

journal_title：Journal of bacteriology

authors： Domenech P,Kolly GS,Leon-Solis L,Fallow A,Reed MB

更新日期：2010-09-01 00:00:00

abstract：:MarR is a key regulator of the marRAB operon involved in antibiotic resistance and solvent stress tolerance in Escherichia coli. We show that two metabolic intermediates, 2,3-dihydroxybenzoate and anthranilate, involved in enterobactin and tryptophan biosynthesis, respectively, can activate marRAB transcription. We al...

journal_title：Journal of bacteriology

authors： Chubiz LM,Rao CV

更新日期：2010-09-01 00:00:00

abstract：:Genetic transformation of Sulfolobus acidocaldarius by a multiply marked pyrE gene provided a high-resolution assay of homologous recombination in a hyperthermophilic archaeon. Analysis of 100 Pyr(+) transformants revealed that this recombination system could transfer each of 23 nonselected base pair substitutions to ...

journal_title：Journal of bacteriology

authors： Grogan DW,Rockwood J

更新日期：2010-09-01 00:00:00

abstract：:Bacillus subtilis, which grows under aerobic conditions, employs fatty acid desaturase (Des) to fluidize its membrane when subjected to temperature downshift. Des requires molecular oxygen for its activity, and its expression is regulated by DesK-DesR, a two-component system. Transcription of des is induced by the tem...

journal_title：Journal of bacteriology

authors： Beranová J,Mansilla MC,de Mendoza D,Elhottová D,Konopásek I

更新日期：2010-08-01 00:00:00

abstract：:Type 1 secretion systems (T1SS) are present in a wide range of Gram-negative bacteria and are involved in the secretion of diverse substrates such as proteases, lipases, and hemophores. T1SS consist of three proteins: an inner membrane ABC (ATP binding cassette) protein, a periplasmic adaptor, and an outer membrane ch...

journal_title：Journal of bacteriology

authors： Masi M,Wandersman C

更新日期：2010-08-01 00:00:00

abstract：:Caulobacter crescentus initiates a single round of DNA replication during each cell cycle. Following the initiation of DNA replication, the essential CckA histidine kinase is activated by phosphorylation, which (via the ChpT phosphotransferase) enables the phosphorylation and activation of the CtrA global regulator. C...

journal_title：Journal of bacteriology

authors： Iniesta AA,Hillson NJ,Shapiro L

更新日期：2010-08-01 00:00:00

abstract：:The reactive alpha-oxoaldehydes such as glyoxal (GO) and methylglyoxal (MG) are generated in vivo from sugars through oxidative stress. GO and MG are believed to be removed from cells by glutathione-dependent glyoxalases and other aldehyde reductases. We isolated a number of GO-resistant (GO(r)) mutants from Escherich...

journal_title：Journal of bacteriology

authors： Lee C,Kim I,Lee J,Lee KL,Min B,Park C

更新日期：2010-08-01 00:00:00

abstract：:The SOS response to DNA damage in bacteria is a well-known component of the complex transcriptional responses to genotoxic environmental stresses such as exposure to reactive oxygen species, alkylating agents, and many of the antibiotics targeting DNA replication. However, bacteria such as Bacillus subtilis also respo...

journal_title：Journal of bacteriology

authors： Hoover SE,Xu W,Xiao W,Burkholder WF

更新日期：2010-08-01 00:00:00

abstract：:The Methanococcus maripaludis energy-conserving hydrogenase B (Ehb) generates low potential electrons required for autotrophic CO(2) assimilation. To analyze the importance of individual subunits in Ehb structure and function, markerless in-frame deletions were constructed in a number of M. maripaludis ehb genes. Thes...

journal_title：Journal of bacteriology

authors： Major TA,Liu Y,Whitman WB

更新日期：2010-08-01 00:00:00

abstract：:Magnetotactic bacteria synthesize specific organelles, the magnetosomes, which are membrane-enveloped crystals of the magnetic mineral magnetite (Fe(3)O(4)). The biomineralization of magnetite involves the uptake and intracellular accumulation of large amounts of iron. However, it is not clear how iron uptake and biom...

journal_title：Journal of bacteriology

authors： Uebe R,Voigt B,Schweder T,Albrecht D,Katzmann E,Lang C,Böttger L,Matzanke B,Schüler D

更新日期：2010-08-01 00:00:00

abstract：:Genome sequences from the prolific Roseobacter clade in the Alphaproteobacteria are beginning to reveal the genetic basis for the diverse lifestyles of these organisms. Here we present the genome sequences of Oceanicola granulosus HTCC2516(T) and Oceanicola batsensis HTCC2597(T), two marine Roseobacter species isolate...

journal_title：Journal of bacteriology

authors： Thrash JC,Cho JC,Vergin KL,Giovannoni SJ

更新日期：2010-07-01 00:00:00

abstract：:Transfer of conjugative plasmids requires relaxases, proteins that cleave one plasmid strand sequence specifically. The F plasmid relaxase TraI (1,756 amino acids) is also a highly processive DNA helicase. The TraI relaxase activity is located within the N-terminal approximately 300 amino acids, while helicase motifs ...

journal_title：Journal of bacteriology

authors： Dostál L,Schildbach JF

更新日期：2010-07-01 00:00:00

abstract：:A genetically homogenous bacterial population may contain physiologically distinct subpopulations. In one such case, a minor part of an otherwise antibiotic-sensitive bacterial population maintains a nondividing state even in a growth-supporting environment and is therefore not killed by bactericidal antibiotics. This...

journal_title：Journal of bacteriology

authors： Jõers A,Kaldalu N,Tenson T

更新日期：2010-07-01 00:00:00

abstract：:Although arsenic is highly toxic to most organisms, certain prokaryotes are known to grow on and respire toxic metalloids of arsenic (i.e., arsenate and arsenite). Two enzymes are known to be required for this arsenic-based metabolism: (i) the arsenate respiratory reductase (ArrA) and (ii) arsenite oxidase (AoxB). Bot...

journal_title：Journal of bacteriology

authors： Zargar K,Hoeft S,Oremland R,Saltikov CW

更新日期：2010-07-01 00:00:00

abstract：:In Shigella flexneri, the polysaccharide copolymerase (PCP) protein Wzz(SF) confers a modal length of 10 to 17 repeat units (RUs) to the O-antigen (Oag) component of lipopolysaccharide (LPS). PCPs form oligomeric structures believed to be related to their function. To identify functionally important regions within Wzz...

journal_title：Journal of bacteriology

authors： Papadopoulos M,Morona R

更新日期：2010-07-01 00:00:00

abstract：:Faithful DNA replication involves the removal of RNA residues from genomic DNA prior to the ligation of nascent DNA fragments in all living organisms. Because the physiological roles of archaeal type 2 RNase H are not fully understood, the substrate structure requirements for the detection of RNase H activity need fur...

journal_title：Journal of bacteriology

authors： Le Laz S,Le Goaziou A,Henneke G

更新日期：2010-07-01 00:00:00

abstract：:Toxin-antitoxin (TA) loci consist of two genes in an operon, encoding a stable toxin and an unstable antitoxin. The expression of toxin leads to cell growth arrest and sometimes bacterial death, while the antitoxin prevents the cytotoxic activity of the toxin. In this study, we show that the chromosome of Yersinia pes...

journal_title：Journal of bacteriology

authors： Goulard C,Langrand S,Carniel E,Chauvaux S

更新日期：2010-07-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli (EHEC) O157:H7 responds to the host-produced epinephrine and norepinephrine, and bacterially produced autoinducer 3 (AI-3), through two-component systems. Further integration of multiple regulatory signaling networks, involving regulators such as the LysR-type transcriptional regulat...

journal_title：Journal of bacteriology

authors： Habdas BJ,Smart J,Kaper JB,Sperandio V

更新日期：2010-07-01 00:00:00

abstract：:Shewanella oneidensis is a metal reducer that uses the cyclic AMP receptor protein, CRP, to regulate anaerobic respiration. In addition, ArcA(So) is required for anaerobic growth with dimethyl sulfoxide (DMSO) and plays a role in aerobic respiration. The sensor kinase that activates ArcA(So) in S. oneidensis is not kn...

journal_title：Journal of bacteriology

authors： Shroff NP,Charania MA,Saffarini DA

更新日期：2010-06-01 00:00:00

abstract：:The bacterial phosphoenolpyruvate phosphotransferase system (PTS) is a highly conserved phosphotransfer cascade that participates in the transport and phosphorylation of selected carbohydrates and modulates many cellular functions in response to carbohydrate availability. It plays a role in the virulence of many bacte...

journal_title：Journal of bacteriology

authors： Houot L,Chang S,Pickering BS,Absalon C,Watnick PI

更新日期：2010-06-01 00:00:00

abstract：:Dehalogenases play key roles in the detoxification of halogenated aromatics. Interestingly, only one hydrolytic dehalogenase for halogenated aromatics, 4-chlorobenzoyl-coenzyme A (CoA) dehalogenase, has been reported. Here, we characterize another novel hydrolytic dehalogenase for a halogenated aromatic compound from ...

journal_title：Journal of bacteriology

authors： Wang G,Li R,Li S,Jiang J

更新日期：2010-06-01 00:00:00

abstract：:Archaeal transcriptional machinery is similar to that of eukaryotes. We studied the fates of various components of the Sulfolobus solfataricus transcriptional apparatus under different stresses and found that in cells incubated at 90 degrees C for 1 h, transcription factor E (TFE) is selectively depleted, but its mRNA...

journal_title：Journal of bacteriology

authors： Iqbal J,Qureshi SA

更新日期：2010-06-01 00:00:00

abstract：:Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen often associated with chronic infections in the lungs of individuals with the heritable disease cystic fibrosis (CF). Previous work from our laboratory demonstrated that aromatic amino acids within CF lung secretions (sputum) not only serve as carbon and...

journal_title：Journal of bacteriology

authors： Palmer GC,Palmer KL,Jorth PA,Whiteley M

更新日期：2010-06-01 00:00:00

abstract：:Sequence analysis of the large virulence plasmid pLVPK in Klebsiella pneumoniae CG43 revealed the presence of another mucoid factor encoding gene rmpA besides rmpA2. Promoter activity measurement indicated that the deletion of rmpA reduced K2 capsular polysaccharide (CPS) biosynthesis, resulting in decreased colony mu...

journal_title：Journal of bacteriology

authors： Cheng HY,Chen YS,Wu CY,Chang HY,Lai YC,Peng HL

更新日期：2010-06-01 00:00:00

abstract：:Streptococcus mutans is a key contributor to the formation of the extracellular polysaccharide (EPS) matrix in dental biofilms. The exopolysaccharides, which are mostly glucans synthesized by streptococcal glucosyltransferases (Gtfs), provide binding sites that promote accumulation of microorganisms on the tooth surfa...

journal_title：Journal of bacteriology

authors： Koo H,Xiao J,Klein MI,Jeon JG

更新日期：2010-06-01 00:00:00

abstract：:The extracellular chlamydial infectious particle, or elementary body (EB), is enveloped by an intra- and intermolecular cysteine cross-linked protein shell called the chlamydial outer membrane complex (COMC). A few abundant proteins, including the major outer membrane protein and cysteine-rich proteins (OmcA and OmcB)...

journal_title：Journal of bacteriology

authors： Liu X,Afrane M,Clemmer DE,Zhong G,Nelson DE

更新日期：2010-06-01 00:00:00

abstract：:In the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, expression of the nitrate assimilation nirA operon takes place in the absence of ammonium and the presence of nitrate or nitrite. Several positive-action proteins that are required for expression of the nirA operon have been identified...

journal_title：Journal of bacteriology

authors： Frías JE,Flores E

更新日期：2010-06-01 00:00:00

abstract：:Human dental biofilm communities comprise several species, which can interact cooperatively or competitively. Bacterial interactions influence biofilm formation, metabolic changes, and physiological function of the community. Lactic acid, a common metabolite of oral bacteria, was measured in the flow cell effluent of ...

journal_title：Journal of bacteriology

authors： Periasamy S,Kolenbrander PE

更新日期：2010-06-01 00:00:00

abstract：:Clinical isolates of the porcine pathogen Actinobacillus pleuropneumoniae often form adherent colonies on agar plates due to expression of an operon, pgaABCD, encoding a poly-beta-1,6-N-acetyl-D-glucosamine (PGA) extracellular matrix. The adherent colony phenotype, which correlates with the ability to form biofilms on...

journal_title：Journal of bacteriology

authors： Bossé JT,Sinha S,Li MS,O'Dwyer CA,Nash JH,Rycroft AN,Kroll JS,Langford PR

更新日期：2010-05-01 00:00:00

abstract：:SigF is an alternative sigma factor that is highly conserved among species of the genus Mycobacterium. In this study we identified the SigF regulon in Mycobacterium smegmatis using whole-genome microarray and promoter consensus analyses. In total, 64 genes in exponential phase and 124 genes in stationary phase are Sig...

journal_title：Journal of bacteriology

authors： Hümpel A,Gebhard S,Cook GM,Berney M

更新日期：2010-05-01 00:00:00

abstract：:The WalRK two-component system plays important roles in maintaining cell wall homeostasis and responding to antibiotic stress in low-GC Gram-positive bacteria. In the major human pathogen, Streptococcus pneumoniae, phosphorylated WalR(Spn) (VicR) response regulator positively controls the transcription of genes encodi...

journal_title：Journal of bacteriology

authors： Gutu AD,Wayne KJ,Sham LT,Winkler ME

更新日期：2010-05-01 00:00:00

abstract：:In the Firmicutes, two-component regulatory systems of the LiaSR type sense and orchestrate the response to various agents that perturb cell envelope functions, in particular lipid II cycle inhibitors. In the current study, we found that the corresponding system in Streptococcus pneumoniae displays similar properties ...

journal_title：Journal of bacteriology

authors： Eldholm V,Gutt B,Johnsborg O,Brückner R,Maurer P,Hakenbeck R,Mascher T,Håvarstein LS

更新日期：2010-04-01 00:00:00

abstract：:Cystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. Initial sequence analysis suggested that Cif is an epoxide hydrolase (EH), but its seque...

journal_title：Journal of bacteriology

authors： Bahl CD,Morisseau C,Bomberger JM,Stanton BA,Hammock BD,O'Toole GA,Madden DR

更新日期：2010-04-01 00:00:00

abstract：:Systematic inactivation of pathways involved in DNA alkylation damage repair demonstrated that inactivation of the ada, ogt, tag, uvrA, and mfd genes is required to detect a Salmonella enterica virulence decrease. Furthermore, the fitness of S. enterica, defective in these genes, is lowered only when the bacterium is ...

journal_title：Journal of bacteriology

authors： Alvarez G,Campoy S,Spricigo DA,Teixidó L,Cortés P,Barbé J

更新日期：2010-04-01 00:00:00

abstract：:Coxiella burnetii is a Gram-negative, obligate intracellular bacterial pathogen that resides within the harsh, acidic confines of a lysosome-like compartment of the host cell that is termed a parasitophorous vacuole. In this study, we characterized a thiol-specific peroxidase of C. burnetii that belongs to the atypica...

journal_title：Journal of bacteriology

authors： Hicks LD,Raghavan R,Battisti JM,Minnick MF

更新日期：2010-04-01 00:00:00

abstract：:The uptake of iron into Pseudomonas aeruginosa is mediated by two major siderophores produced by the bacterium, pyoverdine and pyochelin. The bacterium is also able of utilize several heterologous siderophores of bacterial or fungal origin. In this work, we have investigated the iron uptake in P. aeruginosa PAO1 by th...

journal_title：Journal of bacteriology

authors： Hannauer M,Barda Y,Mislin GL,Shanzer A,Schalk IJ

更新日期：2010-03-01 00:00:00

abstract：:Cells of the gliding bacterium Flavobacterium johnsoniae move rapidly over surfaces. Mutations in gldN cause a partial defect in gliding. A novel bacteriophage selection strategy was used to aid construction of a strain with a deletion spanning gldN and the closely related gene gldO in an otherwise wild-type F. johnso...

journal_title：Journal of bacteriology

authors： Rhodes RG,Samarasam MN,Shrivastava A,van Baaren JM,Pochiraju S,Bollampalli S,McBride MJ

更新日期：2010-03-01 00:00:00

abstract：:In streptococcal species, the key step of competence development is the transcriptional induction of comX, which encodes the alternative sigma factor sigma(X), which positively regulates genes necessary for DNA transformation. In Streptococcus species belonging to the mitis and mutans groups, induction of comX relies ...

journal_title：Journal of bacteriology

authors： Fontaine L,Boutry C,de Frahan MH,Delplace B,Fremaux C,Horvath P,Boyaval P,Hols P

更新日期：2010-03-01 00:00:00

abstract：:Helicobacter pylori is a microaerophilic bacterium associated with gastric inflammation and peptic ulcers. Knowledge of how pathogenic organisms produce energy is important from a therapeutic point of view. We found d-amino acid dehydrogenase-mediated electron transport from d-proline or d-alanine to oxygen via the re...

journal_title：Journal of bacteriology

authors： Tanigawa M,Shinohara T,Nishimura K,Nagata K,Ishizuka M,Nagata Y

更新日期：2010-03-01 00:00:00